Change in activity of serine palmitoyltransferase affects sensitivity to syringomycin E in yeastSaccharomyces cerevisiae
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منابع مشابه
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متن کاملAn improved method to determine serine palmitoyltransferase activity.
Serine palmitoyltransferase (SPT) catalyzes the condensation of l-serine and palmitoyl-CoA, which is the rate-limiting step in the de novo synthesis of sphingolipids. SPT activity is commonly measured by monitoring the incorporation of radiolabeled l-serine into 3-ketodihydrosphingosine. In this article, we introduce several adaptations of the established protocol to improve sensitivity, reprod...
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 2014
ISSN: 0378-1097
DOI: 10.1111/1574-6968.12535